F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, eFluor™ 570, eBioscience™, Invitrogen™

Description

Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding. This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody). Applications Reported: This polyclonal antibody has been reported for use in immunohistochemical staining, and immunocytochemistry. Applications Tested: This polyclonal F(ab')2 Anti-Mouse IgG antibody has been tested by immunocytochemistry after staining with a primary antibody of the mouse isotype. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable.

Mouse IgG (H+L) Polyclonal antibody specifically detects Mouse IgG (H+L) in Mouse samples. It is validated for Immunocytochemistry, Immunohistochemistry.