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Armenian Hamster IgG, Functional Grade, clone: eBio299Arm, Isotype Control, eBioscience™

Armenian Hamster Isotype Control

Manufacturer:  Life Technologies LS16488885

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Catalog No. 501129592



Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: The eBio299Arm antibody has been reported for use as an isotype control for flow cytometry.Applications Tested: This eBio299Arm antibody has been tested by LAL assay to determine endotoxin level and by immunofluorescent staining and flow cytometric analysis of mouse splenocytes and normal human peripheral blood.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

Armenian Hamster IgG
Control
1mg/mL
PBS with no preservative; pH 7.2
IgG
IgG
500μg
Store at 2-8°C.
Monoclonal
Control, Flow Cytometry, Functional Assay
eBio299Arm
Functional Grade
Liquid
Armenian Hamster
Affinity chromatography
RUO
Isotype Control
Not Applicable
Documents

For Research Use Only.