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Armenian Hamster IgG, clone: eBio299Arm, Isotype Control, eBioscience™

Armenian Hamster Isotype Control

Manufacturer:  Life Technologies LS14488885

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Catalog No. 501129491



Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood. Use isotype control at the same concentration as experimental antibody.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Armenian Hamster IgG
Control
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
IgG
IgG
500μg
Store at 2-8°C.
Monoclonal
Control, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
eBio299Arm
Unconjugated
Liquid
Armenian Hamster
Affinity chromatography
RUO
Isotype Control
Not Applicable
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