Thermo Scientific™ IGF-1R (α -Subunit) Ab-3, Mouse Monoclonal Antibody
Provide accurate, reproducible results with the Thermo Scientific™ IGF-1R (α-Subunit) Ab-3, Mouse Monoclonal Antibody.
Manufacturer: Thermo Scientific™ MS643P1ABX
Provide accurate, reproducible results with the Thermo Scientific™ IGF-1R (α-Subunit) Ab-3, Mouse Monoclonal Antibody.IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of α- and Β-subunits, which are disulfide-linked in a Β-α-α-Β configuration in the mature receptor. The α-subunit is completely extracellular, while the Β-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
Host Species: Mouse
Species Reactivity: Human and Rabbit. Weakly reacts with Pig. Does not react with rat.
Epitope: aa 440-514 (exon 7/8)
Immunogen: IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA
Molecular Weight: 125kDa
Positive Control: Placenta or Breast CA
Cellular Localization: Membrane Ab-3 is the antibody-of-choice for blocking binding of IGF-1 to its receptor. It weakly mimics IGF-1 action. Ab-3 reacts only weakly with hybrids of Insulin Receptor and IGF-1R while Ab-5 reacts very well. So, Ab-3, in conjunction with Ab-5, has proved useful in the isolation of hybrid receptors.
- Western Blotting (Not suitable)
- Immunoprecipitation (Not optimum)
- Affinity Purification of Hybrid Receptors
- Acts as a Weak IGF-like Agonist in Bioassays (Thymidine incorporation into DNA)
- Inhibits (>90%) IGF-1 Binding
USA: RUO; Int'l: RUO
|IGF-1R (alpha-Subunit) Ab-3|
|IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA|