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IgD, eFluor 450, Clone: 11-26c (11-26), eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS48599380

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Catalog No. 501129162



Description

Description

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.

Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgD
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Liquid
Ighd
IgG2a, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
380797
Flow Cytometry
11-26c (11-26)
eFluor 450
Ighd
Immunoglobulin D, Ig D
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Documents

For Research Use Only.