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Mouse IFN alpha A Recombinant Protein, Invitrogen™

Mouse IFN alpha A Recombinant Protein, 121001, from Invitrogen™, 121001, from Invitrogen™ Species Reactivity: Mouse; Applications: ELISA, Immunoprecipitation, Western Blot.

Manufacturer:  Pierce Biotechnology Inc PI121001

Catalog No. PI121001


 

Specifications

Description

Mouse IFN alpha A Recombinant Protein, 121001, from Invitrogen., 121001, from Invitrogen. Species Reactivity: Mouse; Applications: ELISA, Immunoprecipitation, Western Blot.

The protein encoded by this gene is produced by macrophages and has antiviral activity.
Host Species: E. Coli
Cross Reactivity: Mouse
Formulation: >1x10^5 units purified protein (Lot-specific) in PBS with 0.1% BSA and no preservative
Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Familletti, et al. (1981), Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394] with the exception that EMCV rather than VSV was used as the challenge. The activity was determined relative to a lab standard of Mu IFN-?A which was calibrated to the NIH mouse IFN-alpha standard (Ga02-901-511). Mouse (L929/EMCV) in this assay the EC50 for IFN is ∽ 5U/ml. Lot Activity was derived from multiple determinations in the above assay. Store at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2x10^5 units/ml is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.
Description & Specifications

Specifications

P01572
Purified
15962
>1x10^5 UN
Store at -80°C, Avoid Freeze/Thaw Cycles
IFNA1
Mouse
Yes
ELISA, Immunoprecipitation, Western Blot
>1x10^5 units purified protein (Lot-specific) in PBS with 0.1% BSA and no preservative
Mouse IFN alpha A
E. coli
Murine interferon Alpha, IFNA
Unlabeled
E. coli