Hygromycin B, 50mg/mL in distilled water, sterile-filtered, Alfa Aesar™
Manufacturer: Thermo Fisher Scientific J673718EQ
Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 μg/ml, MCF-7ALOX12 cells at 150 μg/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.
Fully miscible in water and also soluble in methanol or ethanol and buffer solution.
Protect from heat. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.
|50mg/mL in distilled water|
|5 x 1mL|
Safety and Handling
GHS H Statement
Toxic if swallowed.
Fatal if inhaled.
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Causes serious eye damage.
Harmful in contact with skin.
May cause an allergic skin reaction.
GHS P Statement
Do not breathe dust/fume/gas/mist/vapors/spray.
IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Specific treatment is urgent (see label).
Store locked up.
Dispose of contents/container in accordance with local/regional/national/international regulations.
DOTInformation : Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: TOXIC, LIQUIDS, ORGANIC, N.O.S.
RTECSNumber : WK2130000
storageNote1 : −30° to −10°C