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Hygromycin B, 50mg/mL in distilled water, sterile-filtered, Alfa Aesar™

Manufacturer:  Thermo Fisher Scientific J673718EQ

 View more versions of this product

Catalog No. AAJ673718EQ



Description

Description

Applications
Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 μg/ml, MCF-7ALOX12 cells at 150 μg/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.

Solubility
Fully miscible in water and also soluble in methanol or ethanol and buffer solution.

Notes
Protect from heat. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.
Specifications

Specifications

Hygromycin B
31282-04-9
MFCD06795479
GRRNUXAQVGOGFE-BBMONYMYSA-N
(3'S,3aS,4S,4'S,5'R,6R,6'R,7R,7aS)-4-[(1R,2S,3R,5S,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
134129613
50mg/mL in distilled water
sterile-filtered
C20H37N3O13
UN2810
CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N
527.524
Liquid
5 x 1mL

Chemical Identifiers

31282-04-9
527.524
GRRNUXAQVGOGFE-BBMONYMYSA-N
(3'S,3aS,4S,4'S,5'R,6R,6'R,7R,7aS)-4-[(1R,2S,3R,5S,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
C20H37N3O13
MFCD06795479
134129613
CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N
Safety and Handling

Safety and Handling

GHS H Statement
H301-H330-H334-H318-H312-H317
Toxic if swallowed.
Fatal if inhaled.
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Causes serious eye damage.
Harmful in contact with skin.
May cause an allergic skin reaction.

GHS P Statement
P260-P301+P310-P304+P340-P305+P351+P338-P320-P330-P405-P501a
Do not breathe dust/fume/gas/mist/vapors/spray.
IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Specific treatment is urgent (see label).
Rinse mouth.
Store locked up.
Dispose of contents/container in accordance with local/regional/national/international regulations.

Danger

DOTInformation : Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: TOXIC, LIQUIDS, ORGANIC, N.O.S.

RTECSNumber : WK2130000

storageNote1 : −30° to −10°C

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