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Human VEGF-A ELISA Kits, Thermo Scientific™


Microplate kits for sandwich ELISA of human vascular endothelial growth factor (VEGF)

Manufacturer: pierce biotechnology inc  EH2VEGF5

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Catalog No. ENEH2VEGF5

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Description & Specifications

These human VEGF-A ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of vascular endothelial growth factor (VEGF-A) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant human VEGF-A (vascular endothelial growth factor)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 31.3 to 2000pg/mL
  • Sensitivity: < 5pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 4hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with human VEGF ELISA: human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, PLGF-1, PLGF-2, RANTES, TGFβ, TNFβ, VEGF-B, VEGF-C or VEGF-D; rat VEGF; mouse VEGF120 or VEGF164