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Invitrogen™ TSLP Human Uncoated ELISA Kit with Plates
Specifically engineered for accurate and precise measurement of human TSLP protein levels from samples including serum, plasma, and cell culture supernatants.
Supplier: Invitrogen™ 88749776
Alert:
Contains Azide
Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Description
TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.Specifications
Q969D9 | |
8 pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
BSF-1,IL-4 | |
HRP | |
RUO | |
2°C to 8°C | |
Human | |
24 hr. 30 min. |
8-1,000 pg/mL | |
Biotin | |
Plasma, Serum, Supernatant | |
ELISA | |
85480 | |
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA Coating Buffer Powder: This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86) | |
20 x 96 Tests | |
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL | |
thymic stromal derived lymphopoietin | |
1 hr. 45 min. |
For Research Use Only