Invitrogen™ eBioscience™ Human TSLP ELISA Ready-SET-Go!™ Kit
Specifically engineered for accurate and precise measurement of human TSLP protein levels from samples including serum, plasma, and cell culture supernatants.
Manufacturer: Invitrogen™ LS88749722
DescriptionTSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.
|Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA Coating Buffer Powder: This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)|
|2 x 96 tests|
|Plasma (100μL), Serum (100μL), Supernatants (100μL)|
|24 hr 30 min|
|Colorimetric Microplate Reader|
|Plasma, Serum, Supernatants|
|1 hr 45 min|
|thymic stromal derived lymphopoietin|
For Research Use Only