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eBioscience™ Human TNF alpha, Recombinant Protein

Human Recombinant Protein

$129.05 - $448.05

Specifications

Structural Form E. coli expressed amino acids Val 77-Leu 233 of human TNF- alpha accession # HUMTNFAB, N-terminal His-tag
Purity >95%
Purification Method SDS-PAGE
Molecular Weight 19kDa
Source E. coli
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501128922

 
ebioscience™
14-8329-63
10μg Each for $158.05

501128943

 
ebioscience™
14-8329-62
5μg Each for $129.05

509049

 
ebioscience™
14-8329-81
50μg Each for $448.05
Description & Specifications

Specifications

Structural Form E. coli expressed amino acids Val 77-Leu 233 of human TNF- alpha accession # HUMTNFAB, N-terminal His-tag
Purity >95%
Purification Method SDS-PAGE
Molecular Weight 19kDa
Source E. coli
Accession Number HUMTNFAB
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Formulation Sterile liquid; 10mM sodium phosphate, pH 7.2, 150mM NaCl, 0.5% BSA. 0.22μm filtered.
Biological Activity The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 80pg/mL. This corresponds to a specific activity of greater than or equal to 1.3 x 107 Units/mg.
For Use With (Application) Cytokine Bioassays
Conjugate Unlabeled
Regulatory Status RUO
Cross Reactivity Human
Gene Symbol Tumor Necrosis Factor alpha, TNFa TNF-a
Name TNF alpha
Recombinant Yes

Human TNF-α is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF-α is expressed as a membrane bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-α have been observed.