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Invitrogen™ eBioscience™ Human TNF alpha ELISA Ready-SET-Go!™ Kit

Specifically engineered for accurate and precise measurement of human TNF-α protein levels from samples including serum, plasma, and cell culture supernatants.

Manufacturer:  Invitrogen™ LS88734676

 View more versions of this product

Catalog No. 501125202


Alert:

Contains Azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

Human TNF-α ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA)
Specifications

Specifications

P01375
Biotin
7124
Colorimetric Microplate Reader
ELISA
RUO
Plasma, Serum, Supernatants
Human
1 hr 45 min
TNF alpha
Tumor Necrosis Factor alpha, tnf-a, tnf-alpha
4-500 pg/mL
ELISA
96-well plates
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X), Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
20 x 96 tests
4pg/mL
Plasma (100μL), Serum (100μL), Supernatants (100μL)
2° to 8°C
24 hr 30 min
TNF
HRP
Documents

For Research Use Only