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Thermo Scientific™ anti-Tumor Necrosis Factor Alpha Recombinant, Thermo Scientific™

Tumor Necrosis Factor Alpha Recombinant protein STNFA from Thermo Scientific™ specifically detects Tumor Necrosis Factor Alpha in samples, and it is validated for ELISA and WB

Manufacturer:  Thermo Scientific™ PIEH3TNFA5

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Catalog No. ENEH3TNFA5


 

Specifications

Description

Tumor Necrosis Factor Alpha Recombinant protein STNFA from Thermo Scientific™ specifically detects Tumor Necrosis Factor Alpha in samples, and it is validated for ELISA and WB

These human TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human TNFα (human tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.6 to 1000pg/mL

  • Sensitivity: < 2pg/mL

  • Precision: Dependent upon sample type and level (see instructions)

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, TNFβ, Eotaxin, RANTES, GROα, GROβ, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, GCSF, GMCSF, MIP-1α, MIP-1β, TGFβ, IFNγ, IFNα; mouse TNFα; rat TNFα; and bovine TNFα. TNFα Receptor types 1 and 2 tested at 40mg/mL did not interfere in this assay. Recombinant pig TNFα cross-reacted at 100%

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Description & Specifications

Specifications

<2pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
5 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
5 x 96 microplate wells
Absorbance at 450nm minus absorbance at 550nm
Natural and recombinant human TNFa (human tumor necrosis factor alpha)
Human TNF alpha ELISA Kit
50µL
30X Wash Buffer, 200mL
No significant cross-reaction with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, TNFß, Eotaxin, RANTES, GROa, GROß, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, GCSF, GMCSF, MIP-1a, MIP
15.6 to 1000pg/mL
Dependent upon sample type and level (see instructions)
3.5 hr.
Complete ELISA kit with coated 96-well plates