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Human sTNF RII/TNFRSF1B Quantikine ELISA Kit, R&D Systems

a 3.5 or 4.5 hour solid phase ELISA designed to measure human sTNF RII in cell culture supernates, serum, plasma, or urine.

Manufacturer:  R & D Systems RDSSRT200

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Catalog No. SRT200




Kit, ELISA; R&D Systems; Human sTNF RII/TNFRSF1B Quantikine ELISA Kit; Six 96-well plates; 1 Pk.; Sufficient materials to run ELISAs on at least six 96-well plates

It contains E. coli-expressed recombinant human sTNF RII as well as antibodies raised against this polypeptide. The recombinant protein represents the non-glycosylated, N-terminal methionyl form of the naturally occurring human soluble Type II receptor for TNF, minus 53 amino acids from the proline-rich region of the protein just exterior to the transmembrane domain, with an apparent molecular weight of approximately 19.8 kDa. This immunoassay has been shown to accurately quantitate the recombinant soluble sTNF RII. Results obtained using natural sTNF RII showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural sTNF RII. Since the measurement of human sTNF RII by this immunoassay is insensitive to added TNF-alpha or TNF-beta, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples, (i.e. the total amount of free receptor plus the total amount of receptor bound to TNF)
Description & Specifications


<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.
6 Plates
Cell Culture Supernates (20μL), Serum (20μL), EDTA Plasma (20μL), Heparin Plasma (20μL), Citrate Plasma (20μL), Urine (20μL)
Sufficient materials to run ELISAs on at least six 96-well plates
Solid Phase Sandwich ELISA
1 Pk.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine