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Invitrogen™ Human RANTES ELISA Kits DFS Item

Measure levels of regulated upon activation, normal T-cell expressed and secreted (RANTES) in human serum, plasma, tissue extracts, urine and culture supernatant samples.

Manufacturer:  Invitrogen™ LSEHRNTS5

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Catalog No. ENEHRNTS5



Description

Description

Assay RANTES (regulated upon activation, normal T-cell expressed and secreted) levels in human serum, plasma, tissue extracts, urine and culture supernatant samples with our Invitrgen™ Human RANTES ELISA Kits.

These human RANTES ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of RANTES (regulated upon activation, normal T-cell expressed and secreted) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human RANTES (regulated upon activation, normal T-cell expressed and secreted)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 51.2 to 2000pg/mL

  • Sensitivity: < 2pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human Eotaxin, GROα, GROβ, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNFα or TNFβ, or rat RANTES

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

51.2 to 2000pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
TMB Substrate Solution, 5 x 13mL, Adhesive Plate Covers, 30 ea, Streptavidin-HRP Concentrate, 250µL, 30X Wash Buffer, 200mL, Anti-Human RANTES Precoated 96-well Strip Plate, 5 ea, Biotinylated Antibody Reagent, 35mL, HRP Dilution Buffer, 70mL, Lyophilized Recombinant Human RANTES Standard, 5 vials, Standard Diluent, 110mL, Stop Solution, 55mL
Absorbance at 450nm minus absorbance at 550nm
<2pg/mL
50µL
5 x 96 microplate wells
Natural and recombinant human RANTES (regulated upon activation, normal T-cell expressed and secreted)
Human RANTES ELISA Kit
Complete ELISA kit with coated 96-well plates
Intra-assay CV <10%; inter-assay CV <10%
5 plate kit
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human Eotaxin, GROa, GROß, MIP-1a, MIP-1ß, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNa, IFNg, IL-1a, IL-1ß, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNFa, TNFß, or rat RANTES.
3 hr.
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