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eBioscience™ Human PrimeFlow™ RNA Assay

In situ hybridization assay that combines the power of the ViewRNA™ branched DNA technology with the single cell resolution of flow cytometry. Branched DNA technology uses pairs of target-specific probes to achieve 8,000 to 16,000 fold signal amplification from a desired mRNA transcript.

$2,392.50 - $4,132.50


For Use With (Application) Flow Cytometric Analysis
Storage Requirements Storage conditions vary, see components list for details
Species Human
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Contains Sodium azide and <10% formaldehyde


For Research Use Only

Includes: PrimeFlow™ RNA Tubes
PrimeFlow™ RNA Fixation Buffer 1A
PrimeFlow™ RNA Fixation Buffer 1B
PrimeFlow™ RNA Permeabilization Buffer (10X)
PrimeFlow™ RNA Fixation Buffer 2 (8X)
PrimeFlow™ RNA Wash Buffer
PrimeFlow™ RNA Target Probe Diluent
PrimeFlow™ RNA PreAmp Mix
PrimeFlow™ RNA Amp Mix
PrimeFlow™ RNA Label Probe Diluent
PrimeFlow™ RNA Storage Buffer
Rnase Inhibitor 2 (100X)
PrimeFlow™ RNA Label Probes
Rnase Inhibitor 1 (1000X)
Positive Control Probe Sets:
Type 1 Human RPL13A Alexa Fluor 647
Type 4 Human RPL13A Alexa Fluor 488
Type 6 Human RPL13A Alexa Fluor 750

Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
501125097 Life Technologies
100 tests Each for $4,132.50
501128605 Life Technologies
40 tests Each for $2,392.50


  • Simultaneous detection of up to three RNA targets
  • Combine with cell surface and intracellular staining using fluorochrome-conjugated antibodies to allow further discrimination of specific cell subpopulations
  • This Human PrimeFlow™ RNA Assay kit contains all the reagents needed to conduct the assay, including a set of positive control probes for human ribosomal protein, RPL13A (NM_012423)
  • Target Probe sets for genes of interest are sold separately
  • For the study of mouse samples, use the Mouse PrimeFlow™ RNA Assay (cat. 88-18001)
  • For the study of alternative species, either kit may be used, however, appropriate positive control probes need to be purchased separately.
Description & Specifications


Flow Cytometric Analysis
Storage conditions vary, see components list for details