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Thermo Scientific™ Pierce™ Human MCP-1 ELISA Kits

Measure levels of human monocyte chemoattractant protein-1 (MCP1) in human serum, plasma and cell culture supernatant samples using 96-well plates.

Manufacturer:  Thermo Scientific™ EH2MCP1

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Catalog No. ENEH2MCP1


 

Specifications

Description

Assay MCP-1 (human monocyte chemoattractant protein-1) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human MCP-1 ELISA Kits.

These human MCP-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human MCP-1 (monocyte chemoattractant protein-1)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 25.6 to 1000pg/mL

  • Sensitivity: < 10pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human Angiogenin, EGF, FGF-basic, Eotaxin, GM-CSF, GROα, HB-EGF, HGF, IFNα, IFNγ, KGF, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, PLGF-1, RANTES, TNFα, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, and IL-17

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates

Description & Specifications

Specifications

<10pg/mL
Complete ELISA kit with coated 96-well plate
3 hr.
30X Wash Buffer, 50mL
25.6 to 1000pg/mL
No significant cross-reaction with human Angiogenin, EGF, FGF-basic, Eotaxin, GM-CSF, GROa, HB-EGF, HGF, IFNa, IFNg, KGF, MCP-2, MCP-3, MCP-4, MIP-1a, MIP-1ß, PLGF-1, RANTES, TNFa, IL-1a, IL-1ß, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-1
Human MCP-1 ELISA Kit
50.0µL
Intra-assay CV <10%; inter-assay CV <10%
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Absorbance at 450nm minus absorbance at 550nm
Natural and recombinant human MCP-1 (monocyte chemoattractant protein-1)
96 microplate wells
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)