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Human IL-6 R alpha Quantikine ELISA Kit, R&D Systems

Manufacturer: r & d systems  RDSSR600

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Catalog No. SR600


Description & Specifications


Gene ID (Entrez) 3570
Product Type Solid Phase Sandwich ELISA
Packaging Quantity 6 Plates
Quantity 1 Pk.
Sensitivity 15.1pg/mL
Sample Type Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
Sample Volume Cell Culture Supernates (100μL), Serum (10μL), EDTA Plasma (10μL), Heparin Plasma (10μL), Citrate Plasma (10μL), Urine (50μL)
Species Human
Specificity Natural and recombinant human IL-6 sR
Sufficient For Sufficient materials to run ELISAs on at least six 96-well plates

The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R) and a signal-transducing component of 130 kDa (gp 130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans. This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alphas have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (100μL), Serum (10μL), EDTA Plasma (10μL), Heparin Plasma (10μL), Citrate Plasma (10μL), Urine (50μL)
Sensitivity: 15.1pg/mL
Specificity: Natural and recombinant human IL-6 sR
Cross Reactivity: Cross-species reactivity not tested. <0.5% cross-reactivity observed with available related molecules.