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Human IL-6 Quantikine ELISA Kit, R&D Systems

Manufacturer: r & d systems  RDSD6050

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Catalog No. D6050


Description & Specifications


Gene ID (Entrez) 3569
Product Type Solid Phase Sandwich ELISA
Packaging Quantity 1 Plate
Quantity 1 Kit
Sensitivity 0.7pg/mL
Sample Type Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Sample Volume Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL), Citrate Plasma (100μL)
Species Human
Specificity Natural and recombinant human IL-6
Sufficient For Sufficient materials to run ELISAs on at least one 96-well plate

Interleukin 6 (IL-6), also known as interferon-beta 2, 26 kDa protein and B cell stimulatory factor-2 (BSF-2), is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa - 28 kDa phosphorylated and variably glycosylated molecule. Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL), Citrate Plasma (100μL)
Sensitivity: 0.7pg/mL
Specificity: Natural and recombinant human IL-6
Cross Reactivity: <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.