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Thermo Scientific™ anti-Interleukin 6 Recombinant, Thermo Scientific™


Interleukin 6 Recombinant protein SIL6 from Thermo Scientific™ specifically detects Interleukin 6 in Not Applicable samples, and it is validated for Control, ELISA and WB

Manufacturer: thermo scientific™  ESS0005

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Catalog No. ENESS0005

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Description & Specifications

Specifications

Precision Intra-assay CV <10%; inter-assay CV <10%
Sensitivity <1pg/mL
Kit Contents Human IL-6 Standard, 5 vials
Assay Range 10.2 to 400pg/mL
Quantity 5 plate kit
Sample Size 50µL
Description Human IL-6 ELISA Reagent Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Antibodies and complete reagent set for ELISA
Measurement Absorbance at 450nm minus absorbance at 550nm
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Specificity No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6
Sufficient For 5 x 96 microplate wells
Total Assay Time 3 hr.
Target Natural and recombinant human interleukin-6 (IL-6)

Interleukin 6 Recombinant protein SIL6 from Thermo Scientific™ specifically detects Interleukin 6 in Not Applicable samples, and it is validated for Control, ELISA and WB

These human IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-6 antibody pairs and IL-6 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant human interleukin-6 (IL-6)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 10.2 to 400pg/mL

  • Sensitivity: < 1pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFα, IFNγ, IFNα, GM-CSF, or mouse IL-6

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).