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Thermo Scientific™ Pierce™ Human IL-4 ELISA Kits


Measure levels of human interleukin-4 in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™  EH3IL4

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Catalog No. PIEH3IL4

  • / Pack
 

Description & Specifications

Specifications

Assay Range 10.24 to 400pg/mL
Description Human IL-4 ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
Formulation Complete ELISA kit with coated 96-well plate
Kit Contents 30X Wash Buffer, 50mL
Precision Intra assay CV ≤ 10%; inter-assay CV ≤ 10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 plate kit
Sensitivity ≤ 2pg/mL
Sample Type Cell culture supernatant, serum and plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with human IL-2, IL-6, IL-10, IFNg, GM-CSF or mouse IL-4
Sufficient For 96 microplate wells
Total Assay Time 3.5 hr.
Target Natural and recombinant human interleukin-4 (IL-4; also called BSF-1 or BCGF-1)

Assay IL-4 (human interleukin-4) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human IL-4 ELISA Kits.

The human IL-4 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-4 (IL-4), also known as B-cell stimulatory factor 1 (BSF-1, BSF1) or B-cell growth factor 1 (BCGF-1, BCGF1), in samples at 50μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant human interleukin-4 (IL-4; also called BSF-1 or BCGF-1)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 10.24 to 400pg/mL

  • Sensitivity: ≤ 2pg/mL

  • Precision: Intra assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum and plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-2, IL-6, IL-10, IFNγ, GM-CSF or mouse IL-4

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.