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Thermo Scientific™ Pierce™ Human IL-4 ELISA Kits

Measure levels of human interleukin-4 in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Manufacturer:  Thermo Scientific™ EH3IL4

 View more versions of this product

Catalog No. PIEH3IL4


 

Specifications

Description

Assay IL-4 (human interleukin-4) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human IL-4 ELISA Kits.

The human IL-4 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-4 (IL-4), also known as B-cell stimulatory factor 1 (BSF-1, BSF1) or B-cell growth factor 1 (BCGF-1, BCGF1), in samples at 50μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-4 (IL-4; also called BSF-1 or BCGF-1)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 10.24 to 400pg/mL

  • Sensitivity: ≤ 2pg/mL

  • Precision: Intra assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum and plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-2, IL-6, IL-10, IFNγ, GM-CSF or mouse IL-4

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

≤ 2pg/mL
Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
30X Wash Buffer, 50mL
96 microplate wells
50µL
Cell culture supernatant, serum and plasma (EDTA, sodium citrate and heparin)
3.5 hr.
Absorbance at 450nm minus absorbance at 550nm
Intra assay CV ≤ 10%; inter-assay CV ≤ 10%
10.24 to 400pg/mL
Human IL-4 ELISA Kit
Natural and recombinant human interleukin-4 (IL-4; also called BSF-1 or BCGF-1)
1 plate kit
No significant cross-reaction with human IL-2, IL-6, IL-10, IFNg, GM-CSF or mouse IL-4
Complete ELISA kit with coated 96-well plate