Please login to your online account to display your discounted pricing

Human IL-23 Quantikine ELISA Kit, R&D Systems

$654.17 - $3,135.89

Specifications

Product Type Solid Phase Sandwich ELISA
Gene ID (Entrez) 51561
Sample Volume Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL)
Sensitivity 16.3pg/mL
Species Human
View More Specs
Products
Catalog Number Mfr. No. Packaging Quantity Sufficient For Price Quantity    

D2300B

 
r & d systems
RDSD2300B
1 Plate Sufficient materials to run ELISAs on at least one 96-well plate Each for $654.17

S2300B

 
r & d systems
RDSS2300B
6 Plates Sufficient materials to run ELISAs on at least six 96-well plates Each for $3,135.89
Description & Specifications

Specifications

Product Type Solid Phase Sandwich ELISA
Gene ID (Entrez) 51561
Sample Volume Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL)
Sensitivity 16.3pg/mL
Species Human

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL)
Sensitivity: 16.3pg/mL
Specificity: Natural and recombinant human IL-23
Cross Reactivity: <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.