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Human IL-23 Quantikine ELISA Kit, R&D Systems


Manufacturer: r & d systems  RDSS2300B

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Catalog No. S2300B

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Description & Specifications

Specifications

Gene ID (Entrez) 51561
Product Type Solid Phase Sandwich ELISA
Packaging Quantity 6 Plates
Quantity 1 Pk.
Sensitivity 16.3pg/mL
Sample Type Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sample Volume Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL)
Species Human
Sufficient For Sufficient materials to run ELISAs on at least six 96-well plates

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL)
Sensitivity: 16.3pg/mL
Specificity: Natural and recombinant human IL-23
Cross Reactivity: <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.