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Invitrogen™ eBioscience™ Human IL-21 ELISA Ready-SET-Go!™ Kit, 2nd Generation

Specifically engineered for accurate and precise measurement of IL-21 protein levels from samples including serum, plasma, and cell culture supernatants. This second generation kit has increased sensitivity with a range from 8-1000pg/mL.

Manufacturer:  Invitrogen™ LS88821888

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Catalog No. 501128795


Alert:

Contains Sodium azide


Includes: Capture Antibody.Pre-titrated, purified antibody
Detection Antibody.Pre-titrated, biotin-conjugated antibody
Standard.Recombinant protein for generating standard curve and calibrating samples
Coating Buffer.10X PBS ELISA Coating Buffer
Assay Diluent.5X Concentrated
Detection Enzyme.Pre-titrated Avidin-HRP
Substrate Solution.Tetramethylbenzidine (TMB) Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards.

Description

Description

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.
Specifications

Specifications

Q9HBE4
Biotin
59067
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards., 96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
10 x 96 tests
8 pg/mL
Plasma (100μL), Serum (100μL), Supernatants (100μL)
2-8°C
24 hr 30 min
IL21
HRP
8-1,000 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Plasma, Serum, Supernatants
Human
1 hr 45 min
Human
Interleukin-21
Documents

For Research Use Only