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Human IL-2 Quantikine ELISA Kit, R&D Systems


A 4.5 hour solid phase ELISA designed to measure IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-2 and antibodies raised against recombinant human IL-2.

Manufacturer: r & d systems  RDSS2050

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Catalog No. S2050

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Description & Specifications

Specifications

Sample Type Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Sufficient For Sufficient materials to run ELISAs on at least six 96-well plates
Quantity 1 Pk.
Cross Reactivity <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.
Specificity Natural and recombinant human IL-2
Packaging Quantity 6 Plates
Sensitivity 7pg/mL
Species Human
Product Type Solid Phase Sandwich ELISA
Gene ID (Entrez) 3558
Sample Volume Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL), Citrate Plasma (100μL)

Interleukin 2 (IL-2) was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has also been shown to stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. At the amino acid sequence level, there is approximately 60% - 90% similarity between species. Mature human IL-2 shows 65%, 67%, 72%, 78%, and 64% aa identity to mouse, rat, pig, cat, and cow IL-2, respectively.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL), Citrate Plasma (100μL)
Sensitivity: 7pg/mL
Specificity: Natural and recombinant human IL-2
Cross Reactivity: <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.