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Invitrogen™ PD-L1 Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ LSBMS2212TWO

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Catalog No. 501125368



Description

Description

The Human Programmed T cell Death Ligand 1 (Hu PD-L1) ELISA quantitates Hu PD-L1 in human serum, plasma, cell lysates or cell culture medium. The assay will exclusively recognize both natural and recombinant H PD-L1. Principle of the method The Human PD-L1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
Specifications

Specifications

P60568
Biotin
3558
Pre-coated 96 well plate, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Controls, Chromogen, Stop Solution
2 x 96 tests
9.1 pg/mL
50μL
Human
1 hr 20 min
Human
Aldesleukin, T cell growth factor, TCGF
7%
18.8-1,200 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Plasma, Serum, Supernatants
Plasma (50μL), Serum (50μL), Supernatants (50μL)
2-8°C
3 hr 30 min
IL2
5%
HRP
Documents

For Research Use Only