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Human IL-1ra/IL-1F3 Quantikine ELISA Kit, R&D Systems

Manufacturer: r & d systems  RDSSRA00B

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Catalog No. SRA00B

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Description & Specifications


Gene ID (Entrez) 3557
Product Type Solid Phase Sandwich ELISA
Packaging Quantity 6 Plates
Quantity 1 Pk.
Sensitivity 18.3pg/mL
Sample Type Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sample Volume Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL)
Species Human
Specificity Natural and recombinant human IL-1ra
Sufficient For Sufficient materials to run ELISAs on at least six 96-well plates

IL-1 receptor antagonist (IL-1ra) was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1RII does not bind IL-1ra.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (100μL), Serum (100μL), EDTA Plasma (100μL), Heparin Plasma (100μL)
Sensitivity: 18.3pg/mL
Specificity: Natural and recombinant human IL-1ra
Cross Reactivity: <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.