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Human IL-17E (IL-25) Carrier-Free, Recombinant Protein, eBioscience™

Recombinant Protein

Manufacturer:  Life Technologies LS34873682

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Catalog No. 501129167



Description

Description

IL-17E, or IL-25, was first identified as an IL-17 family member on the basis of sequence homology. It was originally believed to be a Th2 cytokine based on the detection of mRNA transcripts in polarized Th2 cells, but expression was later observed in epithelial cells, eosinophils, and mast cells. Studies have confirmed the role of IL-17E as a potent inducer of type 2 immune responses. IL-17E signaling induces the differentiation of CD4+ T cells towards the Th2 lineage and upregulates expression of Th2 cytokines such as IL-4, IL-5, and IL-13. Administration of IL-17E also promotes symptoms of allergic asthma, such as eosinophil infiltration, airway hyperreactivity, and high circulating levels of IgE. IL-17E binds to IL-17BR, a receptor shared with IL-17B, and activates NF-kappa B pathways. Description: IL-17E, or IL-25, was first identified as an IL-17 family member on the basis of sequence homology. It was originally believed to be a Th2 cytokine based on the detection of mRNA transcripts in polarized Th2 cells, but expression was later observed in epithelial cells, eosinophils, and mast cells. Studies have confirmed the role of IL-17E as a potent inducer of type 2 immune responses. IL-17E signaling induces the differentiation of CD4+ T cells towards the Th2 lineage and upregulates expression of Th2 cytokines such as IL-4, IL-5, and IL-13. Administration of IL-17E also promotes symptoms of allergic asthma, such as eosinophil infiltration, airway hyperreactivity, and high circulating levels of IgE. IL-17E binds to IL-17BR, a receptor shared with IL-17B, and activates NF-kappa B pathways. Applications Reported: This protein (10-100 ng) can to induce IL-8 secretion from normal human peripheral blood cells. Applications Tested: Recombinant IL-17E (IL-25) is biologically active and can promote IL-8 secretion from human PBMCs. Source: E. coli (amino acids Met17-Gly; accession # AAI04930.1). Bioactivity: This protein (10-100 ng) can to induce IL-8 secretion from normal human peripheral blood cells. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC. Molecular Weight: 33.8kDa disulfide-linked homodimer of two 145 aa polypeptide chains. Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water to a concentration of 0.1-1.0mg/mL. Do not vortex.
Specifications

Specifications

Q9H293
Lyophilized
64806
Human IL-17E (IL-25) Carrier-Free
Affinity chromatography
E. coli
RUO
Recombinant
Human IL-17E (IL-25) Carrier-Free
Control, Functional Assay
Human
Hu
Recombinant
Functional Assays
0.01M acetic acid with no preservative
33.8kDa disulfide-linked homodimer of two 145 aa polypeptide chains
SDS-PAGE and HPLC
100μg
-20°C
E. coli (amino acids Met17-Gly; Accession # AAI04930.1)
Less than 0.1ng/μg cytokine as determined by the LAL assay
IL25
This protein (10-100 ng) can to induce IL-8 secretion from normal human peripheral blood cells.
Unlabeled
E. coli
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