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Invitrogen™ IL-13 Human Uncoated ELISA Kit with Plates

Specifically engineered for accurate and precise measurement of human IL-13 protein levels from samples including serum, plasma, and cell culture supernatants.

$385.00 - $994.00

Specifications

Accession Number P35225
Assay Range 4-500 pg/mL
Assay Sensitivity 4 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Alert:

Contains Azide


Products 2
Catalog Number Mfr. No. Quantity Gene Symbol Price Quantity  
Catalog Number Mfr. No. Quantity Gene Symbol Price Quantity  
5017398
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Invitrogen™
88743986
10 x 96 Tests DER4,Fit-1,Ly84,ST2L,St2,St2-rs1,T1,T1/ST2
Each for $994.00
 
501128900
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Invitrogen™
88743922
2 x 96 Tests DER4,Fit-1,Ly84,ST2L,St2,St2-rs1,T1,T1/ST2
Each for $385.00
 
Description

Description

The Human Interleukin 13 (IL-13) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-13. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-13 Human Uncoated ELISA Kit (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA)
Specifications

Specifications

P35225
4 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
DER4,Fit-1,Ly84,ST2L,St2,St2-rs1,T1,T1/ST2
HRP
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Interleukin-13
1 hr. 45 min.
4-500 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
3596
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Streptavidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
RUO
2°C to 8°C
Human
24 hr. 30 min.
SDS
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