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Thermo Scientific™ anti-Interleukin 13 Recombinant, Thermo Scientific™

Interleukin 13 Recombinant protein SIL13 from Thermo Scientific™ specifically detects Interleukin 13 in samples, and it is validated for ELISA and Western Blot

Manufacturer:  Thermo Scientific™ PIEHIL13

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Catalog No. ENEHIL13


 

Specifications

Description

Interleukin 13 Recombinant protein SIL13 from Thermo Scientific™ specifically detects Interleukin 13 in samples, and it is validated for ELISA and Western Blot

These human IL-13 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-13 (IL-13) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-13 (IL-13)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 0 to 1000pg/mL

  • Sensitivity: < 7pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV <10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, TNFα, TGFβ, IFNγ, IFNα, GM-CSF, GROα, GROβ or mouse IL-13

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

0 to 1000pg/mL
Human IL-13 ELISA Kit
Complete ELISA kit with coated 96-well plate
Absorbance at 450nm minus absorbance at 550nm
<7pg/mL
50µL
96 microplate wells
Natural and recombinant human interleukin-13 (IL-13)
30X Wash Buffer, 50mL
Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
Intra-assay CV <10%; inter-assay CV <10%
1 plate kit
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human IL-1a, IL-1ß, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, TNFa, TGFß, IFNg, IFNa, GM-CSF, GROa, GROß or mouse IL-13
3 hr.