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Thermo Scientific™ Pierce™ Human IL-10 ELISA Kits


Measure levels of human interleukin-10 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™  SIL10

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Catalog No. ENSIL10

  • / Each
 

Description & Specifications

Specifications

Sensitivity <3 pg/mL
Precision Intra-assay CV <10%; inter-assay CV <10%
Sufficient For ELISA standard curve with replicates
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Description Human IL-10 Standard for ELISA
Formulation Calibrated to NIBSC reference lot 93/722
Specificity SIL10
Total Assay Time 3 hrs
Assay Range 15.4 to 600 pg/mL
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50 µL
Measurement Absorbance at 450nm minus absorbance at 550nm
Target Natural and recombinant human interleukin-10 (IL-10)
Quantity 1 vial

Assay IL-10 (human interleukin-10) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-10 ELISA Kits.

These human IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant human interleukin-10 (IL-10)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.4 to 600pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ or IFNγ. Approx. 2% cross-reactivity with recombinant mouse IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.