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Human IL-1 beta/IL-1F2 Quantikine ELISA Kit, R&D Systems

a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 beta in cell culture supernates, serum, and plasma.

Manufacturer:  R & D Systems RDSSLB50

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Catalog No. SLB50


 

Specifications

Description

Kit, ELISA; R&D Systems; Human IL-1 beta/IL-1F2 Quantikine ELISA Kit; Six 96-well plates; 1 Pk.; Sufficient materials to run ELISAs on at least six 96-well plates

It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-1 beta accurately. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids
Description & Specifications

Specifications

<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules
3553
6 Plates
1pg/mL
Cell Culture Supernates (200μL), Serum (200μL), EDTA Plasma (200μL), Heparin Plasma (200μL), Citrate Plasma (200μL)
Natural and recombinant human IL-1 beta
ELISA
Solid Phase Sandwich ELISA
1/Pk.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Human
Sufficient materials to run ELISAs on at least six 96-well plates