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Thermo Scientific™ Pierce™ Human IL-1 alpha ELISA Kits

Measure levels of human interleukin-1 alpha (IL-1a) in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

$636.30 - $636.30

Specifications

Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Target Natural and recombinant human interleukin-1 alpha (IL-1a)
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Catalog Number Mfr. No. Quantity Price Quantity    

ENEH2IL1A

 
thermo scientific™
EH2IL1A
1 plate kit Each for $636.30
Description & Specifications

Specifications

Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Target Natural and recombinant human interleukin-1 alpha (IL-1a)

Assay IL-1a (human interleukin-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-1 alpha ELISA Kits.

These human IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant human interleukin-1 alpha (IL-1α)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 10.2 to 400pg/mL

  • Sensitivity: < 2pg/mL

  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IFNα, IFNγ, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, or mouse IL-1α. Human IL-1ra does not interfere with this ELISA

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.