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Invitrogen™ eBioscience™ Human IgA Platinum ELISA Kit
For the quantitative detection of human IgA.
Manufacturer: Invitrogen™ LSBMS2096
DescriptionThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
|Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers|
|20μL (1:10,000 prediluted)|
|1 hr 20 min|
|Colorimetric Microplate Reader|
|Plasma (20μL), Serum (20μL)|
|1 hr 30 min|
For Research Use Only