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Thermo Scientific™ anti-Granulocyte Macrophage Colony Stimulating Factor Recombinant, Thermo Scientific™

Granulocyte Macrophage Colony Stimulating Factor Recombinant protein SGMCSF detects Granulocyte Macrophage Colony Stimulating Factor in Not Applicable samples, and is validated for Control, ELISA and WB

Manufacturer:  Thermo Scientific™ PIEHGMCSF5

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Catalog No. ENEHGMCSF5


 

Specifications

Description

Granulocyte Macrophage Colony Stimulating Factor Recombinant protein SGMCSF detects Granulocyte Macrophage Colony Stimulating Factor in Not Applicable samples, and is validated for Control, ELISA and WB

These human GM-CSF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of granulocyte macrophage colony-stimulating factor (GM-CSF) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.4 to 600pg/mL

  • Sensitivity: < 2pg/mL

  • Reproducibility: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-6, IL-7, IL-8, IFNγ, TNFα or mouse GM-CSF

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Absorbance at 450nm minus absorbance at 550nm
30X Wash Buffer, 200mL
Human GM-CSF ELISA Kit
No significant cross-reaction with human IL-1a, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, IFNg, TNFa or mouse GM-CSF
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
5 x 96 microplate wells
Complete ELISA kit with coated 96-well plates
<2pg/mL
50µL
Natural and recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
5 plate kit
4 hr.
15.4 to 600pg/mL