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Thermo Scientific™ anti-Granulocyte Macrophage Colony Stimulating Factor Recombinant, Thermo Scientific™


Granulocyte Macrophage Colony Stimulating Factor Recombinant protein SGMCSF detects Granulocyte Macrophage Colony Stimulating Factor in Not Applicable samples, and is validated for Control, ELISA and WB

Manufacturer: thermo scientific™  EHGMCSF

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Catalog No. ENEHGMCSF

  • / Each
 

Description & Specifications

Specifications

Assay Range 15.4 to 600pg/mL
Description Human GM-CSF ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plate
Kit Contents 30X Wash Buffer, 50mL
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 plate kit
Reproducibility Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sensitivity <2pg/mL
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with human IL-1a, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, IFNg, TNFa or mouse GM-CSF
Sufficient For 96 microplate wells
Total Assay Time 4 hr.
Target Natural and recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)

Granulocyte Macrophage Colony Stimulating Factor Recombinant protein SGMCSF detects Granulocyte Macrophage Colony Stimulating Factor in Not Applicable samples, and is validated for Control, ELISA and WB

These human GM-CSF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of granulocyte macrophage colony-stimulating factor (GM-CSF) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.4 to 600pg/mL

  • Sensitivity: < 2pg/mL

  • Reproducibility: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-6, IL-7, IL-8, IFNγ, TNFα or mouse GM-CSF

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.