eBioscience™ Human Fc Receptor Binding Inhibitor, Purified
Can be used to inhibit the non-specific Fc-gamma receptor (FcγR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue.
Manufacturer: Life Technologies LS14916173
DescriptionA test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.
|Fc Receptor Binding Inhibitor|
|Flow Cytometric Analysis|
|100 tests,100 Tests|
|Fc blocking; FcR Inhibitor; Fc Receptor Inhibitor; FcR BI|
|Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|2° to 8°C|
For Research Use Only