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Thermo Scientific™ Pierce™ Human EGF ELISA Kits

Measure levels of epidermal growth factor (EGF) in human serum, plasma, and cell culture supernatant using 96-well plates.

Manufacturer:  Thermo Scientific™ EHEGF

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Catalog No. ENEHEGF


 

Specifications

Description

Assay EGF (epidermal growth factor) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Human EGF ELISA Kits.

These human EGF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of epidermal growth factor (EGF, also called URG, HOMG4) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched EGF antibody pairs and EGF standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human epidermal growth factor (EGF); also known as urogastrone (URG), hypomagnesemia type 4 (HOMG4)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 7.8 to 500pg/mL

  • Sensitivity: ≤ 5pg/mL

  • Reproducibility: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human HB-EGF (Heparin-binding EGF), mouse EGF, rat EGF and human IL-2, IL-4, IL-6, IL-8, TNFα TGFβ, GM-CSF

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Description & Specifications

Specifications

Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human HB-EGF (Heparin-binding EGF), mouse EGF, rat EGF and human IL-2, IL-4, IL-6, IL-8, TNFa TGFß, GM-CSF
96 microplate wells
7.8 to 500pg/mL
Complete ELISA kit with coated 96-well plate
Absorbance at 450nm minus absorbance at 550nm
≤ 5pg/mL
30X Wash Buffer, 50mL
Human EGF ELISA Kit
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
1 plate kit
Natural and recombinant human epidermal growth factor (EGF); also known as urogastrone (URG), hypomagnesemia type 4 (HOMG4)
50µL