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Human Cytochrome c Quantikine ELISA Kit, R&D Systems

a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma.

Manufacturer:  R & D Systems RDSSCTC0

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Catalog No. SCTC0


 

Specifications

Description

Kit, ELISA; R&D Systems; Human Cytochrome c Quantikine ELISA Kit; Six 96-well plates; 1 Pk.; Sufficient materials to run ELISAs on at least six 96-well plates

This assay employs the quantitative sandwich enzyme immunoassay technique and is designed to replace a Western Blot. A monoclonal antibody specific for human Cytochrome c has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cytochrome c present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for human Cytochrome c is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cytochrome c bound in the initial step. The color development is stopped and the intensity of the color is measured.
Description & Specifications

Specifications

<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules
54205
6 Plates
0.31ng/mL
Cell Lysates (20μL), Subcellular Fractions (100μL)
Sufficient materials to run ELISAs on at least six 96-well plates
ELISA
Solid Phase Sandwich ELISA
1 Pk.
Cell Lysates, Subcellular Fractions
Human