Human Cytochrome c Quantikine ELISA Kit, R&D Systems
a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma.
Manufacturer: R & D Systems RDSSCTC0
Kit, ELISA; R&D Systems; Human Cytochrome c Quantikine ELISA Kit; Six 96-well plates; 1 Pk.; Sufficient materials to run ELISAs on at least six 96-well platesThis assay employs the quantitative sandwich enzyme immunoassay technique and is designed to replace a Western Blot. A monoclonal antibody specific for human Cytochrome c has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cytochrome c present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for human Cytochrome c is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cytochrome c bound in the initial step. The color development is stopped and the intensity of the color is measured.
|<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules|
|Cell Lysates (20μL), Subcellular Fractions (100μL)|
|Sufficient materials to run ELISAs on at least six 96-well plates|
|Solid Phase Sandwich ELISA|
|Cell Lysates, Subcellular Fractions|