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Human GM-CSF Animal Free, Recombinant Protein, eBioscience™

Recombinant Protein

Manufacturer:  Life Technologies LS68877763

 View more versions of this product

Catalog No. 501124903



Description

Description

Granulocyte Macrophage Colony Stimulating Factor, GM-CSF, is hematopoietic factor produced by endothelial cells, monocytes, fibroblasts and T cells in response to a number of inflammatory mediators. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. Human and mouse GM-CSF show no cross-reactivity. Description: This SAFE™ (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin. Human GM-CSF (Granulocyte/Monocyte-Colony Stimulating Factor) is a differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF alpha, IL-1, IL-2 and IFN gamma. GM-CSF is found associated with extracellular matrix and in membrane-bound formats too. GM-CSF stimulates proliferation, activation and differentiation of macrophages and granulocytes and their progenitors. Applications Reported: SAFE™ Human GM-CSF Recombinant Animal Free Protein is biologically active. Applications Tested: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than 0.2ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg. Source: Plant expressed 127 amino acids (Ala18-Glu144). accession NP_000749.2. Bioactivity: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than 0.2ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg. Endotoxin: Less than 0.005ng/ug (0.05 EU/ug) as measured by turbidity assay. Purity: Greater than or equal to 95% as determined by SDS-PAGE. Molecular Weight: 26kDa with glycosylation. Storage: The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile reagent grade deionized water to a concentration no less than 100 ug/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Specifications

Specifications

P04141
Lyophilized
1437
Human GM-CSF Animal Free
SDS-PAGE
10μg
4°C or -20°C if preferred
Plant expressed 127 amino acids (Ala18-Glu144). Accession NP_000749.2
Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay
CSF2
The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than 0.2ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 106 Units/mg.
Human
Hu
Recombinant
Functional Assays
PBS with no preservative; pH 7.2
26kDa with glycosylation
7.2
Affinity chromatography
Plant
RUO
Recombinant
Human GM-CSF Animal Free
Control, Functional Assay
Recombinant Protein
Unlabeled
Plant
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