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Human CCL3/MIP-1 alpha Quantikine ELISA Kit, R&D Systems

Manufacturer: r & d systems  RDSSMA00

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Catalog No. SMA00


Description & Specifications


Gene ID (Entrez) 6348
Product Type Solid Phase Sandwich ELISA
Packaging Quantity 6 Plates
Quantity 1 Pk.
Sensitivity 10pg/mL
Sample Type Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Sample Volume Cell Culture Supernates (10μL), Serum (200μL), EDTA Plasma (200μL), Heparin Plasma (200μL), Citrate Plasma (200μL)
Species Human
Sufficient For Sufficient materials to run ELISAs on at least six 96-well plates

MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.

The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (10μL), Serum (200μL), EDTA Plasma (200μL), Heparin Plasma (200μL), Citrate Plasma (200μL)
Sensitivity: 10pg/mL
Specificity: Natural and recombinant human MIP-1 alpha
Cross Reactivity: <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.