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Invitrogen™ Caspase 9 Human ELISA Kit
For the quantitative detection of human Caspase-9.
$752.00 - $5,240.00
Specifications
Accession Number | P55211 |
---|---|
Assay Range | 1.6-100 ng/mL |
Assay Sensitivity | 0.4 ng/mL |
Conjugate | HRP |
Product Type | ELISA Kit |
Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-9
Anti-human Caspase-9 polyclonal Detection Antibody (rabbit)
Anti-rabbit-IgG-HRP
Human Caspase-9 Standard lyophilized, 200ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Lysis Buffer 10x
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Description
The Human Caspase 9 ELISA quantitates Hu Caspase 9 in human serum, cell lysate or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Caspase 9. Principle of the method The Human Caspase 9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.Specifications
P55211 | |
0.4 ng/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
APAF-3,APAF3,ICE-LAP6,MCH6,PPP1R56 | |
6.6% | |
HRP | |
Cell Lysate,50 μL; Serum, 50 μL; Supernatant, 50 μL | |
Apoptotic protease MCH-6, APAF-3, ICE-LAP6 | |
1 hr. 20 min. |
1.6-100 ng/mL | |
HRP | |
Cell Lysates, Serum, Supernatant | |
ELISA | |
842 | |
9% | |
Pre-coated 96 well plate, Standard, Standard, Assay Buffer concentrate, Assay Buffer concentrate, anti-rabbit HRP conjugate, anti-rabbit HRP conjugate, Lysis Buffer, Chromogen, Chromogen, Stop Solution | |
RUO | |
2°C to 8°C | |
Human | |
3 hr. 10 min. |
For Research Use Only