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Invitrogen™ eBioscience™ Human C5a Platinum ELISA Kit

For the detection of human C5a in serum, plasma (EDTA, citrate, heparin) and cell culture supernatant

Manufacturer:  Invitrogen™ LSBMS2088

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Catalog No. 501125291



Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human C5a

Description

Description

C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9). The molecular mass of C5 is 190 kDa.
Specifications

Specifications

P01031
Biotin
727
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 tests
0.005 ng/mL
Sample volume 10μL for plasma and 5μL for serum (prediluted 1:100 for serum, 1:25 for plasma), 50μL for CCS
Human
1 hr 20 min
Human
complement component 5, C5a, C5D, C5b, ECLZB, CPAMD4
3.6%
0.078-5.0 ng/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Plasma, Serum, Supernatants
Plasma (10μL), Serum (5μL), Supernatants (50μL)
2-8°C
4 hr 30 min
C5
4%
HRP
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