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Human Free BDNF Quantikine ELISA Kit, R&D Systems

$667.02 - $3,201.43

Specifications

Product Type Solid Phase Sandwich ELISA
Gene ID (Entrez) 627
Sample Type Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma
Sample Volume Cell Culture Supernates (50μL), Serum (10μL), EDTA Plasma (50μL), Platelet-poor Heparin Plasma (50μL), Citrate Plasma (50μL)
Sensitivity 20pg/mL
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Products
Catalog Number Mfr. No. Packaging Quantity Sufficient For Price Quantity    

DBD00

 
r & d systems
RDSDBD00
1 Plate Sufficient materials to run ELISAs on at least one 96-well plate Each for $667.02

SBD00

 
r & d systems
SBD00
6 Plates Sufficient materials to run ELISAs on at least six 96-well plates Each for $3,201.43
Description & Specifications

Specifications

Product Type Solid Phase Sandwich ELISA
Gene ID (Entrez) 627
Sample Type Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma
Sample Volume Cell Culture Supernates (50μL), Serum (10μL), EDTA Plasma (50μL), Platelet-poor Heparin Plasma (50μL), Citrate Plasma (50μL)
Sensitivity 20pg/mL
Species Human

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (50μL), Serum (10μL), EDTA Plasma (50μL), Platelet-poor Heparin Plasma (50μL), Citrate Plasma (50μL)
Sensitivity: 20pg/mL
Specificity: Natural and recombinant human BDNF
Cross Reactivity: <50% cross-species reactivity observed with species tested. Cross-reactivity observed with 1 or more available related molecules.