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Human Free BDNF Quantikine ELISA Kit, R&D Systems

Manufacturer: r & d systems  SBD00

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Catalog No. SBD00


Description & Specifications


Gene ID (Entrez) 627
Product Type Solid Phase Sandwich ELISA
Packaging Quantity 6 Plates
Quantity 1 Pk.
Sensitivity 20pg/mL
Sample Type Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma
Sample Volume Cell Culture Supernates (50μL), Serum (10μL), EDTA Plasma (50μL), Platelet-poor Heparin Plasma (50μL), Citrate Plasma (50μL)
Species Human
Sufficient For Sufficient materials to run ELISAs on at least six 96-well plates

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (50μL), Serum (10μL), EDTA Plasma (50μL), Platelet-poor Heparin Plasma (50μL), Citrate Plasma (50μL)
Sensitivity: 20pg/mL
Specificity: Natural and recombinant human BDNF
Cross Reactivity: <50% cross-species reactivity observed with species tested. Cross-reactivity observed with 1 or more available related molecules.