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HLA-ABC Mouse anti-Human, Biotin, Clone: W6/32, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS13998382

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Catalog No. 501128896



Description

Description

Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey. Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis. Applications Tested: This W6/32 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. maligt cells may lose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.
Specifications

Specifications

HLA-ABC
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P05534
H-2K, H-2K(d), H2-K, MGC184092, MGC7052
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
W6/32
Biotin
HLA-A
Liquid
HLA-A
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
3105
Documents

For Research Use Only.