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Cytiva HiTrap™ rProtein A FF Columns

Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. Cytiva Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.

$608.70 - $5024.58

Specifications

Binding Capacity ≈50 mg Human IgG/mL
For Use With (Application) Chromatography System, Manual
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Content And Storage 4°C to 30°C, 20% Ethanol
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Products 4
Catalog Number Mfr. No. Quantity Flow Rate Price Quantity  
Catalog Number Mfr. No. Quantity Flow Rate Price Quantity  
45-000-262
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Cytiva
17507901
5 x 1 mL <4 mL/min.
Pack of 5 Each for $1,257.88
 
45-000-263
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Cytiva
17507902
2 x 1 mL <4 mL/min.
Pack of 2 Each for $608.70
 
45-000-265
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Cytiva
17508002
5 x 5 mL <20 mL/min.
Pack of 5 Each for $5,024.58
 
45-000-264
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Cytiva
17508001
1 x 5 mL <20 mL/min.
Each for $1,228.42
 
Description

Description

  • Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
  • Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
  • Average particle size: 90μm
  • Designed for use with a syringe, a peristaltic pump, or a chromatography system
  • Available as 1mL and 5mL columns
Media
  • Ligand: Recombinant protein A (E.coli)
  • Matrix: Highly cross-linked 4% agarose
  • Binding capacity: ∽50mg human IgG/mL
  • pH stability working range: 3 to 10

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.
5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Order Info

1mL and 5mL columns are packed with 1mL and 5mL of rProtein A Sepharose Fast Flow, respectively.

Specifications

Specifications

≈50 mg Human IgG/mL
Chromatography System, Manual
For syringe, peristaltic pump or a chromatography system
4°C to 30°C, 20% Ethanol
SDS
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