Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads
Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.
Manufacturer: Thermo Scientific™ PI88832*
Perform affinity purification of His-tagged fusion proteins in manual or automated formats with Thermo Scientific™ HisPur Ni-NTA Magnetic Beads, high capacity nickel-IMAC beads.
High capacity – equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers
Low nonspecific binding – the bead surface is pre-blocked and the protocol provides optimized buffers for purification
Fast – protocol is completed in 1 hour
Scalable – process microliter to milliliter sample volumes
Versatile – purify proteins using native or denaturing conditions
Reagent compatible – can be used with common cell lysis reagents and a variety of buffer additives
Multiple formats – protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific™ KingFisher Instruments)
|HisPur Ni-NTA Magnetic Beads|
|Nickel on nitrilotriacetic acid covalently coupled to blocked superparamagnetic beads; supplied at 12.5mg/mL in 20% ethanol|
|Binding at least 500µg green fluorescent protein (GFP)/mL of bead|