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Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads

Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.

Manufacturer:  Thermo Scientific™ PI88831

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Catalog No. PI88831




Perform affinity purification of His-tagged fusion proteins in manual or automated formats with Thermo Scientific™ HisPur Ni-NTA Magnetic Beads, high capacity nickel-IMAC beads.

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The blocked magnetic bead surface is derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) beads provide high binding capacity with very low background. The HisPur Ni–NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high–throughput needs.


  • High capacity – equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers

  • Low nonspecific binding – the bead surface is pre-blocked and the protocol provides optimized buffers for purification

  • Fast – protocol is completed in 1 hour

  • Scalable – process microliter to milliliter sample volumes

  • Versatile – purify proteins using native or denaturing conditions

  • Reagent compatible – can be used with common cell lysis reagents and a variety of buffer additives

  • Multiple formats – protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific™ KingFisher Instruments)

Description & Specifications


HisPur Ni-NTA Magnetic Beads
Nickel on nitrilotriacetic acid covalently coupled to blocked superparamagnetic beads; supplied at 12.5mg/mL in 20% ethanol
Binding at least 500µg green fluorescent protein (GFP)/mL of bead