Thermo Scientific™ HisProbe-HRP is an nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP). This product has been optimized for direct detection of recombinant histidine-tagged proteins and other histidine-rich proteins.Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC).
Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies
High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags
Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies
|Store HisProbe-HRP, Blocker BSA in TBS (10X), and Surfact-Amps 20 at 4°C. All other components may be stored at room temperature.|